P2Y₂ and P2X4 Receptors Mediate Ca²⁺ Mobilization in DH82 Canine Macrophage Cells.

ABSTRACT: Purinergic receptors of the P2 subclass are commonly found in human and rodent macrophages where they can be activated by adenosine 5'-triphosphate (ATP) or uridine 5'-triphosphate (UTP) to mediate Ca²⁺ mobilization, resulting in downstream signalling to promote inflammation and pain. However, little is understood regarding these receptors in canine macrophages. To establish a macrophage model of canine P2 receptor signalling, the expression of these receptors in the DH82 canine macrophage cell line was determined by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry. P2 receptor function in DH82 cells was pharmacologically characterised using nucleotide-induced measurements of Fura-2 AM-bound intracellular Ca²⁺. RT-PCR revealed predomina

SUBMITTER: Sophocleous RA 

PROVIDER: S-EPMC7696671 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature