Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli.

ABSTRACT: Signal transduction utilizing membrane-spanning receptors and cytoplasmic regulator proteins is a fundamental process for all living organisms, but quantitative studies of the behavior of signaling proteins, such as their diffusion within a cell, are limited. In this study, we show that fluctuations in the concentration of the signaling molecule, phosphorylated CheY, constitute the basis of chemotaxis signaling. To analyze the propagation of the CheY-P signal quantitatively, we measured the coordination of directional switching between flagellar motors on the same cell. We analyzed the time lags of the switching of two motors in both CCW-to-CW and CW-to-CCW switching (∆tCCW-CW and ∆tCW-CCW). In wild-type cells, both time lags increased as a function of the relative distance of two motors f

SUBMITTER: Che YS 

PROVIDER: S-EPMC7696710 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature