Cryopreservation of Mesenchymal Stem Cells Using Medical Grade Ice Nucleation Inducer.

ABSTRACT: Mesenchymal stem cells (MSCs) can differentiate into multiple different tissue lineages and have favourable immunogenic potential making them an attractive prospect for regenerative medicine. As an essential part of the manufacturing process, preservation of these cells whilst maintaining potential is of critical importance. An uncontrolled area of storage remains the rate of change of temperature during freezing and thawing. Controlled-rate freezers attempted to rectify this; however, the change of phase from liquid to solid introduces two extreme phenomena; a rapid rise and a rapid fall in temperature in addition to the intended cooling rate (normally -1 °C/min) as a part of the supercooling event in cryopreservation. Nucleation events are well known to initiate the freezing transition a

SUBMITTER: Wragg NM 

PROVIDER: S-EPMC7696797 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature