MicroRNA-146a suppresses tumor malignancy via targeting vimentin in esophageal squamous cell carcinoma cells with lower fibronectin membrane assembly.

ABSTRACT:

Background

Esophageal squamous cell carcinoma (ESCC) is widely prevalent in Taiwan, and high metastatic spread of ESCC leads to poor survival rate. Fibronectin (FN) assembly on the cell membrane may induce ESCC mobility. MicroRNAs (MiRNAs) are abundant in and participate in tumorigenesis in many cancers. However, the role of MiRNA in FN assembly-related ESCC mobility remains unexplored.

Methods

We divided ESCC CE81T cells into high-FN assembly (CE81^FN+) and low-FN assembly (CE81^FN-) groups by flow cytometry. MiRNA microarray analysis identified miR-146a expression as the most down-regulated miRNA in comparison of CE81^FN+ and CE81^FN- cells.

Results

Cell proliferation and migration were decreased when CE81^FN+ cells

SUBMITTER: Chang HY 

PROVIDER: S-EPMC7697386 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature