Combined Effects of Confinement and Macromolecular Crowding on Protein Stability.

ABSTRACT: Confinement and crowding have been shown to affect protein fates, including folding, functional stability, and their interactions with self and other proteins. Using both theoretical and experimental studies, researchers have established the independent effects of confinement or crowding, but only a few studies have explored their effects in combination; therefore, their combined impact on protein fates is still relatively unknown. Here, we investigated the combined effects of confinement and crowding on protein stability using the pores of agarose hydrogels as a confining agent and the biopolymer, dextran, as a crowding agent. The addition of dextran further stabilized the enzymes encapsulated in agarose; moreover, the observed increases in enhancements (due to the addition of dextran) ex

SUBMITTER: Ross ML 

PROVIDER: S-EPMC7697604 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature