Identification of Novel Yellow Fever Class II Epitopes in YF-17D Vaccinees.

ABSTRACT: Yellow fever virus (YFV) is a mosquito-borne member of the genus flavivirus, including other important human-pathogenic viruses, such as dengue, Japanese encephalitis, and Zika. Herein, we report identifying 129 YFV Class II epitopes in donors vaccinated with the live attenuated YFV vaccine (YFV-17D). A total of 1156 peptides predicted to bind 17 different common HLA-DRB1 allelic variants were tested using IFNγ ELISPOT assays in vitro re-stimulated peripheral blood mononuclear cells from twenty-six vaccinees. Overall, we detected responses against 215 YFV epitopes. We found that the capsid and envelope proteins, as well as the non-structural (NS) proteins NS3 and NS5, were the most targeted proteins by CD4⁺ T cells from YF-VAX vaccinated donors. In addition, we designed and vali

SUBMITTER: Mateus J 

PROVIDER: S-EPMC7697718 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature