Single Amino Acid Mutations Affect Zika Virus Replication In Vitro and Virulence In Vivo.

ABSTRACT: The 2014-2016 Zika virus (ZIKV) epidemic in the Americas resulted in large deposits of next-generation sequencing data from clinical samples. This resource was mined to identify emerging mutations and trends in mutations as the outbreak progressed over time. Information on transmission dynamics, prevalence, and persistence of intra-host mutants, and the position of a mutation on a protein were then used to prioritize 544 reported mutations based on their ability to impact ZIKV phenotype. Using this criteria, six mutants (representing naturally occurring mutations) were generated as synthetic infectious clones using a 2015 Puerto Rican epidemic strain PRVABC59 as the parental backbone. The phenotypes of these naturally occurring variants were examined using both cell culture and murine mode

SUBMITTER: Collette NM 

PROVIDER: S-EPMC7697975 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature