Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells.

ABSTRACT: Coxsackie B (CVB) viruses have been associated with type 1 diabetes. We have recently observed that CVB1 was linked to the initiation of the autoimmune process leading to type 1 diabetes in Finnish children. Viral persistency in the pancreas is currently considered as one possible mechanism. In the current study persistent infection was established in pancreatic ductal and beta cell lines (PANC-1 and 1.1B4) using four different CVB1 strains, including the prototype strain and three clinical isolates. We sequenced 5' untranslated region (UTR) and regions coding for structural and non-structural proteins and the second single open reading frame (ORF) protein of all persisting CVB1 strains using next generation sequencing to identify mutations that are common for all of these strains. One mut

SUBMITTER: Honkimaa A 

PROVIDER: S-EPMC7697981 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature