Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection.

ABSTRACT: Studies of virus-host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropi

SUBMITTER: Koban R 

PROVIDER: S-EPMC7698278 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature