Nutritional Conditions Modulate C. neoformans Extracellular Vesicles' Capacity to Elicit Host Immune Response.

ABSTRACT: Cryptococcus neoformans is a human pathogenic fungus that mainly afflicts immunocompromised patients. One of its virulence strategies is the production of extracellular vesicles (EVs), containing cargo with immunomodulatory properties. We evaluated EV's characteristics produced by capsular and acapsular strains of C. neoformans (B3501 and ΔCap67, respectively) growing in nutritionally poor or rich media and co-cultures with bone marrow-derived macrophages or dendritic cells from C57BL/6 mice. EVs produced under a poor nutritional condition displayed a larger hydrodynamic size, contained more virulence compounds, and induced a more robust inflammatory pattern than those produced in a rich nutritional medium, independently of strain. We treated infected mice with EVs produced i

SUBMITTER: Marina CL 

PROVIDER: S-EPMC7698703 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature