<i>Anaplasma phagocytophilum</i> and Other <i>Anaplasma</i> spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa.

ABSTRACT: DNA samples from 74 patients with non-malarial acute febrile illness (AFI), 282 rodents, 100 cattle, 56 dogs and 160 Rhipicephalus sanguineus ticks were screened for the presence of Anaplasma phagocytophilum DNA using a quantitative PCR (qPCR) assay targeting the msp2 gene. The test detected both A. phagocytophilum and Anaplasma sp. SA/ZAM dog DNA. Microbiome sequencing confirmed the presence of low levels of A. phagocytophilum DNA in the blood of rodents, dogs and cattle, while high levels of A. platys and Anaplasma sp. SA/ZAM dog were detected in dogs. Directed sequencing of the 16S rRNA and gltA genes in selected samples revealed the presence of A. phagocytophilum DNA in humans, dogs and rodents and highlighted its im

SUBMITTER: Kolo AO 

PROVIDER: S-EPMC7698776 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature