In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells.

ABSTRACT: Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells' viability with and without calcium administration. For high-voltage pulses, the cell death's mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer

SUBMITTER: Kielbik A 

PROVIDER: S-EPMC7699241 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature