A multiplexed, electrochemical interface for gene-circuit-based sensors.

ABSTRACT: The field of synthetic biology has used the engineered assembly of synthetic gene networks to create a wide range of functions in biological systems. To date, gene-circuit-based sensors have primarily used optical proteins (for example, fluorescent, colorimetric) as reporter outputs, which has limited the potential to measure multiple distinct signals. Here we present an electrochemical interface that permits expanded multiplexed reporting for cell-free gene-circuit-based sensors. We have engineered a scalable system of reporter enzymes that cleave specific DNA sequences in solution, which results in an electrochemical signal when these newly liberated strands are captured at the surface of a nanostructured microelectrode. We describe the development of this interface and show its utility

SUBMITTER: Sadat Mousavi P 

PROVIDER: S-EPMC7700015 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature