Three-Dimensional Biofabrication Models of Endometriosis and the Endometriotic Microenvironment.

ABSTRACT: Endometriosis occurs when endometrial-like tissue grows outside the uterine cavity, leading to pelvic pain, infertility, and increased risk of ovarian cancer. The present study describes the optimization and characterization of cellular spheroids as building blocks for Kenzan scaffold-free method biofabrication and proof-of-concept models of endometriosis and the endometriotic microenvironment. The spheroid building blocks must be of a specific diameter (~500 μm), compact, round, and smooth to withstand Kenzan biofabrication. Under optimized spheroid conditions for biofabrication, the endometriotic epithelial-like cell line, 12Z, expressed high levels of estrogen-related genes and secreted high amounts of endometriotic inflammatory factors that were independent of TNFα stimulation. Heterot

SUBMITTER: Wendel JRH 

PROVIDER: S-EPMC7700676 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature