Measurement of length distribution of beta-lactoglobulin fibrils by multiwavelength analytical ultracentrifugation.

ABSTRACT: The whey protein beta-lactoglobulin is the building block of amyloid fibrils which exhibit a great potential in various applications. These include stabilization of gels or emulsions. During biotechnological processing, high shear forces lead to fragmentation of fibrils and therefore to smaller fibril lengths. To provide insight into such processes, pure straight amyloid fibril dispersions (prepared at pH 2) were produced and sheared using the rotor stator setup of an Ultra Turrax. In the first part of this work, the sedimentation properties of fragmented amyloid fibrils sheared at different stress levels were analyzed with mulitwavelength analytical ultracentrifugation (AUC). Sedimentation data analysis was carried out with the boundary condition that fragmented fibrils were of cylindrica

SUBMITTER: Uttinger MJ 

PROVIDER: S-EPMC7701075 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature