Dataset Information

Mapping choline metabolites in normal and transformed cells.

ABSTRACT:

Introduction

Choline is an essential human nutrient that is particular important for proliferating cells, and altered choline metabolism has been associated with cancer transformation. Yet, the various metabolic fates of choline in proliferating cells have not been investigated systematically.

Objectives

This study aims to map the metabolic products of choline in normal and cancerous proliferating cells.

Methods

We performed ¹³C-choline tracing followed by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis of metabolic products in normal and in vitro-transformed (tumor-forming) epithelial cells, and also in tumor-derived cancer cell lines. Selected metabolites were quantified by internal standards.