Purification and characterisation of new laccase from Trametes polyzona WRF03.

ABSTRACT: The molecular screening for laccase specific gene sequences in Trametes polyzona WRF03 (TpWRF03) using designed oligonucleotide primers analogous to the conserved sequences on the copper-binding regions of known laccases showed positive amplification with an amplicon size corresponding to 1500 bp. The purified TpWRF03 laccase (TpL) is a monomer with a molecular weight corresponding to 66 kDa. The enzyme had an optimal pH of 4.5 and temperature of 55 °C. TpL was most stable within pH of 5.5-6.5 and at a temperature range of 40-50 °C. Sodium azide, sodium cyanide and Fe²⁺ greatly inhibited the enzyme activity. TpL showed more than 50 % decolourisation efficiency on coomassie brilliant blue (72.35 %) and malachite green (57.84 %) but displa

SUBMITTER: Ezike TC 

PROVIDER: S-EPMC7701954 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature