Cancer biology functional genomics: From small RNAs to big dreams.

ABSTRACT: The year 2021 marks the 20th anniversary of the first publications reporting the discovery of the gene silencing mechanism, RNA interference (RNAi) in mammalian cells. Along with the many studies that delineated the proteins and substrates that form the RNAi pathway, this finding changed our understanding of the posttranscriptional regulation of mammalian gene expression. Furthermore, the development of methods that exploited the RNAi pathway began the technological revolution that eventually enabled the interrogation of mammalian gene function-from a single gene to the whole genome-in only a few days. The needs of the cancer research community have driven much of this progress. In this perspective, we highlight milestones in the development and application of RNAi-based methods to study c

SUBMITTER: Sundara Rajan S 

PROVIDER: S-EPMC7702050 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature