Validating a predictive model of cannabinoid inheritance with feral, clinical, and industrial Cannabis sativa.

ABSTRACT:

Premise

How genetic variation within a species affects phytochemical composition is a fundamental question in botany. The ratio of two specialized metabolites in Cannabis sativa, tetrahydrocannabinol (THC) and cannabidiol (CBD), can be grouped into three main classes (THC-type, CBD-type, and intermediate type). We tested a genetic model associating these three groups with functional and nonfunctional alleles of the cannabidiolic acid synthase gene (CBDAS).

Methods

We characterized cannabinoid content and assayed CBDAS genotypes of >300 feral C. sativa plants in Minnesota, United States. We performed a test cross to assess CBDAS inheritance. Twenty clinical cultivars obtained blindly from the National Institute on Drug Abuse and 12 Canadian-certified grain cultivars were also

SUBMITTER: Wenger JP 

PROVIDER: S-EPMC7702092 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature