DNA methylation analysis with methylation-sensitive high-resolution melting (MS-HRM) reveals gene panel for glioma characteristics.

ABSTRACT:

Introduction

Local DNA hypermethylation is a potential source of cancer biomarkers. While the evaluation of single gene methylation has limited value, their selected panel may provide better information.

Aims

This study aimed to analyze the promoter methylation level in a 7-gene panel in brain tumors and verifies the usefulness of methylation-sensitive high-resolution melting (MS-HRM) for this purpose.

Methods

Forty-six glioma samples and one non-neoplastic brain sample were analyzed by MS-HRM in terms of SFRP1, SFRP2, RUNX3, CBLN4, INA, MGMT, and RASSF1A promoter methylation. The results were correlated with patients' clinicopathological features.

Results

DNA methylation level of all analyzed genes was significantly higher in brain tumor samples as compared to

SUBMITTER: Majchrzak-Celinska A 

PROVIDER: S-EPMC7702229 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature