Overexpression of DoUGP Enhanced Biomass and Stress Tolerance by Promoting Polysaccharide Accumulation in Dendrobium officinale.

ABSTRACT: Uridine diphosphate glucose pyrophosphorylase (UDP-glucose pyrophosphorylase, UGPase), as one of the key enzymes in polysaccharide synthesis, plays important roles in the growth and development of plants. In this study, the DoUGP gene of Dendrobium officinale was overexpressed. The expression of DoUGP and genes playing roles in the same and other saccharide synthesis pathways was determined, and the total soluble polysaccharide was also tested in wild-type and transgenic seedlings. We also performed freezing and osmotic stress treatments to determine whether overexpression of DoUGP could influence stress resistance in transgenic seedlings. Results showed that mRNA expression levels of DoUGP and its metabolic upstream and downstream genes in the transgenic

SUBMITTER: Chen J 

PROVIDER: S-EPMC7703667 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature