Proteome-level assessment of origin, prevalence and function of leucine-aspartic acid (LD) motifs.

ABSTRACT:

Motivation

Leucine-aspartic acid (LD) motifs are short linear interaction motifs (SLiMs) that link paxillin family proteins to factors controlling cell adhesion, motility and survival. The existence and importance of LD motifs beyond the paxillin family is poorly understood.

Results

To enable a proteome-wide assessment of LD motifs, we developed an active learning based framework (LD motif finder; LDMF) that iteratively integrates computational predictions with experimental validation. Our analysis of the human proteome revealed a dozen new proteins containing LD motifs. We found that LD motif signalling evolved in unicellular eukaryotes more than 800 Myr ago, with paxillin and vinculin as core constituents, and nuclear export signal as a likely source of de novo LD motifs.

SUBMITTER: Alam T 

PROVIDER: S-EPMC7703752 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature