Identification of the enzymes responsible for m2,2G and acp3U formation on cytosolic tRNA from insects and plants.

ABSTRACT: Posttranscriptional modification of tRNA is critical for efficient protein translation and proper cell growth, and defects in tRNA modifications are often associated with human disease. Although most of the enzymes required for eukaryotic tRNA modifications are known, many of these enzymes have not been identified and characterized in several model multicellular eukaryotes. Here, we present two related approaches to identify the genes required for tRNA modifications in multicellular organisms using primer extension assays with fluorescent oligonucleotides. To demonstrate the utility of these approaches we first use expression of exogenous genes in yeast to experimentally identify two TRM1 orthologs capable of forming N2,N2-dimethylguanosine (m2,2G) on residue 26 of cytosolic tRNA in the mo

SUBMITTER: Funk HM 

PROVIDER: S-EPMC7704012 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature