Reduced reticulum-mitochondria Ca²⁺ transfer is an early and reversible trigger of mitochondrial dysfunctions in diabetic cardiomyopathy.

ABSTRACT: Type 2 diabetic cardiomyopathy features Ca²⁺ signaling abnormalities, notably an altered mitochondrial Ca²⁺ handling. We here aimed to study if it might be due to a dysregulation of either the whole Ca²⁺ homeostasis, the reticulum-mitochondrial Ca²⁺ coupling, and/or the mitochondrial Ca²⁺ entry through the uniporter. Following a 16-week high-fat high-sucrose diet (HFHSD), mice developed cardiac insulin resistance, fibrosis, hypertrophy, lipid accumulation, and diastolic dysfunction when compared to standard diet. Ultrastructural and proteomic analyses of cardiac reticulum-mitochondria interface revealed tighter interactions not compatible with Ca²⁺ transport in HFHSD cardiomyocytes. Intramyocardial adenoviral injections of C

SUBMITTER: Dia M 

PROVIDER: S-EPMC7704523 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature