A molecular cell atlas of the human lung from single-cell RNA sequencing.

ABSTRACT: Although single-cell RNA sequencing studies have begun to provide compendia of cell expression profiles^1-9, it has been difficult to systematically identify and localize all molecular cell types in individual organs to create a full molecular cell atlas. Here, using droplet- and plate-based single-cell RNA sequencing of approximately 75,000 human cells across all lung tissue compartments and circulating blood, combined with a multi-pronged cell annotation approach, we create an extensive cell atlas of the human lung. We define the gene expression profiles and anatomical locations of 58 cell populations in the human lung, including 41 out of 45 previously known cell types and 14 previously unknown ones. This comprehensive molecular atlas identifies the biochemical functions of lu

SUBMITTER: Travaglini KJ 

PROVIDER: S-EPMC7704697 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature