Simultaneous cortex-wide fluorescence Ca²⁺ imaging and whole-brain fMRI.

ABSTRACT: Achieving a comprehensive understanding of brain function requires multiple imaging modalities with complementary strengths. We present an approach for concurrent widefield optical and functional magnetic resonance imaging. By merging these modalities, we can simultaneously acquire whole-brain blood-oxygen-level-dependent (BOLD) and whole-cortex calcium-sensitive fluorescent measures of brain activity. In a transgenic murine model, we show that calcium predicts the BOLD signal, using a model that optimizes a gamma-variant transfer function. We find consistent predictions across the cortex, which are best at low frequency (0.009-0.08 Hz). Furthermore, we show that the relationship between modality connectivity strengths varies by region. Our approach links cell-type-specific optical measure

SUBMITTER: Lake EMR 

PROVIDER: S-EPMC7704940 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature