Mutant thermal proteome profiling for characterization of missense protein variants and their associated phenotypes within the proteome.

ABSTRACT: Temperature-sensitive (TS) missense mutants have been foundational for characterization of essential gene function. However, an unbiased approach for analysis of biochemical and biophysical changes in TS missense mutants within the context of their functional proteomes is lacking. We applied MS-based thermal proteome profiling (TPP) to investigate the proteome-wide effects of missense mutations in an application that we refer to as mutant thermal proteome profiling (mTPP). This study characterized global impacts of temperature sensitivity-inducing missense mutations in two different subunits of the 26S proteasome. The majority of alterations identified by RNA-Seq and global proteomics were similar between the mutants, which could suggest that a similar functiona

SUBMITTER: Peck Justice SA 

PROVIDER: S-EPMC7705321 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature