TagSeq for gene expression in non-model plants: A pilot study at the Santa Rita Experimental Range NEON core site.

ABSTRACT:

Premise

TagSeq is a cost-effective approach for gene expression studies requiring a large number of samples. To date, TagSeq studies in plants have been limited to those with a high-quality reference genome. We tested the suitability of reference transcriptomes for TagSeq in non-model plants, as part of a study of natural gene expression variation at the Santa Rita Experimental Range National Ecological Observatory Network (NEON) core site.

Methods

Tissue for TagSeq was sampled from multiple individuals of four species (Bouteloua aristidoides and Eragrostis lehmanniana [Poaceae], Tidestromia lanuginosa [Amaranthaceae], and Parkinsonia florida [Fabaceae]) at two locations on three dates (56 samples total). One sample per species was used to create

SUBMITTER: Marx HE 

PROVIDER: S-EPMC7705334 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature