Hyperpolarized NMR Metabolomics at Natural ¹³C Abundance.

ABSTRACT: Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on ¹H 1D spectroscopy, severely limited by peak overlap. ¹³C NMR benefits from a larger signal dispersion but is barely used in metabolomics due to ca. 6000-fold lower sensitivity. We introduce a new approach, based on hyperpolarized ¹³C NMR at natural abundance, that circumvents this limitation. A new untargeted NMR-based metabolomic workflow based on dissolution dynamic nuclear polarization (d-DNP) for the first time enabled hyperpolarized natural abundance ¹³C metabolomics. Statistical analysis of resulting hyperpolarized ¹³C data distinguishes

SUBMITTER: Dey A 

PROVIDER: S-EPMC7705890 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature