Comparative transcriptomic analysis of antimony resistant and susceptible Leishmania infantum lines.

ABSTRACT:

Background

One of the major challenges to leishmaniasis treatment is the emergence of parasites resistant to antimony. To study differentially expressed genes associated with drug resistance, we performed a comparative transcriptomic analysis between wild-type and potassium antimonyl tartrate (Sb^III)-resistant Leishmania infantum lines using high-throughput RNA sequencing.

Methods

All the cDNA libraries were constructed from promastigote forms of each line, sequenced and analyzed using STAR for mapping the reads against the reference genome (L. infantum JPCM5) and DESeq2 for differential expression statistical analyses. All the genes were functionally annotated using sequence similarity search.

Results

The analytical pipeline considering an adjusted p-value

SUBMITTER: Andrade JM 

PROVIDER: S-EPMC7706067 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature