Small Molecule-Inducible RNA-Targeting Systems for Temporal Control of RNA Regulation.

ABSTRACT: All aspects of mRNA lifetime and function, including its stability, translation into protein, and trafficking through the cell, are tightly regulated through coordinated post-transcriptional modifications and interactions with a multitude of RNA effector proteins. Despite the increasing recognition of RNA regulation as a critical layer of mammalian gene expression control and its increasing excitement as a therapeutic target, tools to study and control RNA regulatory mechanisms with temporal precision in their endogenous environment are lacking. Here, we present small molecule-inducible RNA-targeting effectors based on our previously developed CRISPR/Cas-inspired RNA targeting system (CIRTS). The CIRTS biosensor platform is based on guide RNA (gRNA)-dependent RNA binding domains that inter

SUBMITTER: Rauch S 

PROVIDER: S-EPMC7706094 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature