Deep sampling and pooled amplicon sequencing reveals hidden genic variation in heterogeneous rye accessions.

ABSTRACT:

Background

Loss of genetic variation negatively impacts breeding efforts and food security. Genebanks house over 7 million accessions representing vast allelic diversity that is a resource for sustainable breeding. Discovery of DNA variations is an important step in the efficient use of these resources. While technologies have improved and costs dropped, it remains impractical to consider resequencing millions of accessions. Candidate genes are known for most agronomic traits, providing a list of high priority targets. Heterogeneity in seed stocks means that multiple samples from an accession need to be evaluated to recover available alleles. To address this we developed a pooled amplicon sequencing approach and applied it to the out-crossing cereal rye (Secale cereale L.).

Resu

SUBMITTER: Hawliczek A 

PROVIDER: S-EPMC7706248 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature