Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans.

ABSTRACT: Histone H3.3 is a replication-independent variant of histone H3 with important roles in development, differentiation, and fertility. Here, we show that loss of H3.3 results in replication defects in Caenorhabditis elegans embryos at elevated temperatures. To characterize these defects, we adapt methods to determine replication timing, map replication origins, and examine replication fork progression. Our analysis of the spatiotemporal regulation of DNA replication shows that despite the very rapid embryonic cell cycle, the genome is replicated from early and late firing origins and is partitioned into domains of early and late replication. We find that under temperature stress conditions, additional replication origins become activated. Moreover, loss of H3.3 results in altered repl

SUBMITTER: Strobino M 

PROVIDER: S-EPMC7706726 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature