Integrative proteomics reveals principles of dynamic phosphosignaling networks in human erythropoiesis.

ABSTRACT: Human erythropoiesis is an exquisitely controlled multistep developmental process, and its dysregulation leads to numerous human diseases. Transcriptome and epigenome studies provided insights into system-wide regulation, but we currently lack a global mechanistic view on the dynamics of proteome and post-translational regulation coordinating erythroid maturation. We established a mass spectrometry (MS)-based proteomics workflow to quantify and dynamically track 7,400 proteins and 27,000 phosphorylation sites of five distinct maturation stages of in vitro reconstituted erythropoiesis of CD34⁺ HSPCs. Our data reveal developmental regulation through drastic proteome remodeling across stages of erythroid maturation encompassing most protein classes. This includes various orchestrat

SUBMITTER: Karayel O 

PROVIDER: S-EPMC7706838 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature