Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics.

ABSTRACT: Ebola virus (EBOV) causes epidemics with high mortality yet remains understudied due to the challenge of experimentation in high-containment and outbreak settings. Here, we used single-cell transcriptomics and CyTOF-based single-cell protein quantification to characterize peripheral immune cells during EBOV infection in rhesus monkeys. We obtained 100,000 transcriptomes and 15,000,000 protein profiles, finding that immature, proliferative monocyte-lineage cells with reduced antigen-presentation capacity replace conventional monocyte subsets, while lymphocytes upregulate apoptosis genes and decline in abundance. By quantifying intracellular viral RNA, we identify molecular determinants of tropism among circulating immune cells and examine temporal dynamics in viral and host gene expression.

SUBMITTER: Kotliar D 

PROVIDER: S-EPMC7707107 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature