Identification of unusual phospholipids from bovine heart mitochondria by HPLC-MS/MS.

ABSTRACT: Phospholipids, including ether phospholipids, are composed of numerous isomeric and isobaric species that have the same backbone and acyl chains. This structural resemblance results in similar fragmentation patterns by collision-induced dissociation of phospholipids regardless of class, yielding complicated MS/MS spectra when isobaric species are analyzed together. Furthermore, the presence of isobaric species can lead to misassignment of species when made solely based on their molecular weights. In this study, we used normal-phase HPLC for ESI-MS/MS analysis of phospholipids from bovine heart mitochondria. Class separation by HPLC eliminates chances for misidentification of isobaric species from different classes of phospholipids. Chromatography yields simple MS/MS spectra without interfe

SUBMITTER: Kim J 

PROVIDER: S-EPMC7707168 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature