Nuclear translocation ability of Lipin differentially affects gene expression and survival in fed and fasting Drosophila.

ABSTRACT: Lipins are eukaryotic proteins with functions in lipid synthesis and the homeostatic control of energy balance. They execute these functions by acting as phosphatidate phosphatase enzymes in the cytoplasm and by changing gene expression after translocation into the cell nucleus, in particular under fasting conditions. Here, we asked whether nuclear translocation and the enzymatic activity of Drosophila Lipin serve essential functions and how gene expression changes, under both fed and fasting conditions, when nuclear translocation is impaired. To address these questions, we created a Lipin null mutant, a mutant expressing Lipin lacking a nuclear localization signal (Lipin^ΔNLS ), and a mutant expressing enzymatically dead Lipin. Our data support the conclusio

SUBMITTER: Hood SE 

PROVIDER: S-EPMC7707171 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature