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Characterization of the complete mitochondrial genome of Ageratum conyzoides.

ABSTRACT: As a pharmaceutical plant with multi-bioactivity, Ageratum conyzoides appears to be a valuable agricultural resource. In this study, the complete mitochondrial (mt) genome of A. conyzoides was sequenced through Illumina sequencing method, and the mt genome was recovered after de novo assembly and annotation. The results showed that the 219,198 bp mt genome has a total of 52 genes, including 30 protein-coding genes, 3 rRNA genes and 19 tRNA genes. The overall GC content of this mitogenome is 45.4%. By phylogenetic analysis using maximum-likelihood (ML) method, A. conyzoides showed the closest relationship with Diplostephium hartwegii in the family of Asteroideae.

SUBMITTER: Luo ZP

PROVIDER: S-EPMC7707261 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Characterization of the complete mitochondrial genome of Ageratum conyzoides.

Luo Ze-Ping ZP Pan Li-Wei LW

Mitochondrial DNA. Part B, Resources 20191012 2

As a pharmaceutical plant with multi-bioactivity, Ageratum conyzoides appears to be a valuable agricultural resource. In this study, the complete mitochondrial (mt) genome of A. conyzoides was sequenced through Illumina sequencing method, and the mt genome was recovered after de novo assembly and annotation. The results showed that the 219,198 bp mt genome has a total of 52 genes, including 30 protein-coding genes, 3 rRNA genes and 19 tRNA genes. The overall GC content of th ...[more]

PMID: 33366076

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Project description:BACKGROUND:Giardia duodenalis causes giardiasis in humans, particularly in developing countries. Despite the availability of treatments, resistance to some of the commercial anti-Giardia drugs has been reported in addition to their harmful side effects. Therefore, novel treatments for giardiasis are required. In this study, we aimed to assess the in vitro activity of crude extracts of Ageratum conyzoides against G. duodenalis trophozoites. METHODS:Plants were classified into three groups based on their flower colors: white (W), purple (P), and white-purple (W-P). Plants were separately cut into leaf (L) and flower (F) parts. Changes in internal organelle morphology of trophozoites following exposure to crude extracts were assessed using transmission electron microscopy (TEM). In subsequent experiments, efficacy of the most active essential oils from crude extracts [half maximal inhibitory concentrations (IC50) ≤ 100 μg/mL] against G. duodenalis trophozoites was tested. In vitro anti-Giardia assays using essential oils were performed in the same way as those performed using crude extracts. RESULTS:LW-P and FP extracts showed high activity (IC50 ≤ 100 μg/mL) against G. duodenalis trophozoites, with IC50 ± SD values of 45.67 ± 0.51 and 96.00 ± 0.46 μg/mL, respectively. In subsequent experiments, IC50 ± SD values of LW-P and FP essential oils were 35.00 ± 0.50 and 89.33 ± 0.41 μg/mL, respectively. TEM revealed the degeneration of flagella and ventral discs of G. duodenalis trophozoites following exposure to crude extracts. CONCLUSION:Crude LW-P and FP extracts of A. conyzoides showed the highest activity against G. duodenalis. Exposure to crude extract induced changes in the flagella and ventral discs of G. duodenalis trophozoites, which play important roles in attachment to the surface of mucosal cells. Our results suggest that the tested extracts warrant further research in terms of their efficacy and safety as giardiasis treatment.

Project description:Ageratum conyzoides L., an invasive plant originating from South America, is characterized by rapid growth and strong ecological adaptability, posing a threat to China's ecosystems, agricultural industry, and biodiversity. In this study, we optimized the MaxEnt model using the ENMeval package and constructed an ensemble model using the Biomod2 package based on global geospatial distribution data of A. conyzoides and considering climate, soil, and topography factors. We simulated the potential suitable distribution of A. conyzoides in China at present and in the future (2041-2060, 2061-2080). Through multivariate environment similarity surface and most dissimilar variable analysis, we identified the main environmental variables influencing the distribution of A. conyzoides. Additionally, niche analysis elucidated temporal and spatial variations in A. conyzoides' climate niche. Our results demonstrate that the ensemble model, constructed from the top seven single models, outperforms the individual models in predicting the suitable habitat of A. conyzoides. The ensemble model achieved the true skill statistic (TSS) of 0.833 and the area under the subject curve (AUC) of 0.971, indicative of outstanding predictive performance. Presently, the suitable habitat of A. conyzoides in China primarily exists in the region between 18° and 28° N, covering approximately 1.47 million km2. The temperature annual range, precipitation of the wettest month, and mean temperature of the coldest quarter were identified as the primary environmental variables influencing its distribution, while soil and elevation variables had minor roles. Under future climate conditions, the suitable habitat of A. conyzoides is expected to expand northeastward, with the centroid of its habitat shifting northward as the climate warms. The migration speed of A. conyzoides is projected to increase with the degree of warming. Furthermore, the climate niche of A. conyzoides will undergo certain changes and may face both niche expansion and a decrease in niche overlap under different climate conditions.

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Characterization of the complete mitochondrial genome of Ageratum conyzoides.

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Characterization of the complete mitochondrial genome of <i>Ageratum conyzoides</i>.

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