Dataset Information

The complete mitochondrial genome sequencing of Anisakis simplex isolated from Anoplopoma fimbria.

ABSTRACT: In this study, zoonotic Anisakis simplex was isolated and identified from the outermost layer of the stomach of diseased Anoplopoma fimbria at an industrial farm in Liaoning, North China (122.1842 E, 39.2616 N). With the completion of A. simplex mitochondrial genome sequencing, the full-length mitochondrial genome of A. simplex was assembled and analyzed. All results indicate that the complete mitochondrial genome of A. simplex was 13,899 bp. There were 20 tRNAs and 12 protein-coding genes (PCGs), and two rRNA all located at the heavy (H) strand. Besides, the phylogenetic tree of 19 A. simplex isolated from different host species was constructed. The results showed that A. simplex isolated from A. fimbria was clustered with Oncorhynchus nerka isolate in a clade. To sum up, our research results would further provide essential data for systematics and evolution study of A. simplex.

SUBMITTER: Wang M

PROVIDER: S-EPMC7707344 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome sequencing of Anisakis simplex isolated from Anoplopoma fimbria.

Wang Maolin M Gao Yanqi Y Li Xuejie X Wang Wei W Li Ruijun R

Mitochondrial DNA. Part B, Resources 20191004 2

In this study, zoonotic Anisakis simplex was isolated and identified from the outermost layer of the stomach of diseased Anoplopoma fimbria at an industrial farm in Liaoning, North China (122.1842 E, 39.2616 N). With the completion of A. simplex mitochondrial genome sequencing, the full-length mitochondrial genome of A. simplex was assembled and analyzed. All results indicate that the complete mitochondrial genome of A. simplex was 13,899 bp. There were 20 tRNA ...[more]

PMID: 33365978

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Project description:BackgroundThe sablefish (order: Scorpaeniformes) is an economically important species in commercial fisheries of the North Pacific and an emerging species in aquaculture. Aside from a handful of sequences in NCBI and a few published microsatellite markers, little is known about the genetics of this species. The development of genetic tools, including polymorphic markers and a linkage map will allow for the successful development of future broodstock and mapping of phenotypes of interest. The significant sexual dimorphism between females and males makes a genetic test for early identification of sex desirable.ResultsA full mitochondrial genome is presented and the resulting phylogenetic analysis verifies the placement of the sablefish within the Scorpaeniformes. Nearly 35,000 assembled transcript sequences are used to identify genes and obtain polymorphic SNP and microsatellite markers. 360 transcribed polymorphic loci from two sablefish families produce a map of 24 linkage groups. The sex phenotype maps to sablefish LG14 of the male map. We show significant conserved synteny and conservation of gene-order between the threespine stickleback Gasterosteus aculeatus and sablefish. An additional 1843 polymorphic SNP markers are identified through next-generation sequencing techniques. Sex-specific markers and sequence insertions are identified immediately upstream of the gene gonadal-soma derived factor (gsdf), the master sex determinant locus in the medaka species Oryzias luzonensis.ConclusionsThe first genomic resources for sablefish provide a foundation for further studies. Over 35,000 transcripts are presented, and the genetic map represents, as far as we can determine, the first linkage map for a member of the Scorpaeniformes. The observed level of conserved synteny and comparative mapping will allow the use of the stickleback genome in future genetic studies on sablefish and other related fish, particularly as a guide to whole-genome assembly. The identification of sex-specific insertions immediately upstream of a known master sex determinant implicates gsdf as an excellent candidate for the master sex determinant for sablefish.

Dataset Information

The complete mitochondrial genome sequencing of Anisakis simplex isolated from Anoplopoma fimbria.

Publications

The complete mitochondrial genome sequencing of <i>Anisakis simplex</i> isolated from <i>Anoplopoma fimbria</i>.

Similar Datasets

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