3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice.

ABSTRACT: Out of millions of ejaculated sperm, a few reach the fertilization site in mammals. Flagellar Ca²⁺ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection. Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca²⁺ and phosphorylation cascades. CatSper1 processing correlates with protein tyrosine phosphorylation (pY) development in sperm cells capacitated in vitro and in vivo. Using 3D in situ molecular imaging and ANN-based automatic detection of sperm distributed along the cleared female tract, we demonstrate

SUBMITTER: Ded L 

PROVIDER: S-EPMC7707823 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature