TRNA import across the mitochondrial inner membrane in T. brucei requires TIM subunits but is independent of protein import.

ABSTRACT: Mitochondrial tRNA import is widespread, but mechanistic insights of how tRNAs are translocated across mitochondrial membranes remain scarce. The parasitic protozoan T. brucei lacks mitochondrial tRNA genes. Consequently, it imports all organellar tRNAs from the cytosol. Here we investigated the connection between tRNA and protein translocation across the mitochondrial inner membrane. Trypanosomes have a single inner membrane protein translocase that consists of three heterooligomeric submodules, which all are required for import of matrix proteins. In vivo depletion of individual submodules shows that surprisingly only the integral membrane core module, including the protein import pore, but not the presequence-associated import motor are required for mitochondrial tRNA import. Thus we co

SUBMITTER: Shikha S 

PROVIDER: S-EPMC7708065 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature