A thermostable bacterial lytic polysaccharide monooxygenase with high operational stability in a wide temperature range.

ABSTRACT:

Background

Lytic polysaccharide monooxygenases (LPMOs) are oxidative, copper-dependent enzymes that function as powerful tools in the turnover of various biomasses, including lignocellulosic plant biomass. While LPMOs are considered to be of great importance for biorefineries, little is known about industrial relevant properties such as the ability to operate at high temperatures. Here, we describe a thermostable, cellulose-active LPMO from a high-temperature compost metagenome (called mgLPMO10).

Results

MgLPMO10 was found to have the highest apparent melting temperature (83 °C) reported for an LPMO to date, and is catalytically active up to temperatures of at least 80 °C. Generally, mgLPMO10 showed good activity and operational stability over a wide temperature range. The L

SUBMITTER: Tuveng TR 

PROVIDER: S-EPMC7708162 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature