Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS.

ABSTRACT:

Background

We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS). To find biomarkers of calcium deficiency and further confirm these biomarkers in serum, we performed serum metabolomics analysis of calcium-deficient rats.

Methods

A calcium-deficient rat model was established with a low-calcium diet for 12 weeks. Serum metabolomics based UPLC/Q-TOF MS/MS and multivariate statistical analysis was performed to identify the alterations in metabolites associated with calcium deficiency in rats.

Results

Bone mineral density, serum parathyroid hormone and alkaline phosphatase were significantly decreased in the low-

SUBMITTER: Meng F 

PROVIDER: S-EPMC7708254 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature