Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system.

ABSTRACT:

Background

Elaboration of the epigenetic regulation of chromatin is a long-standing aim in molecular and cellular biology. Hence, there is a great demand for the development of in vitro methods to reconstitute chromatin that can be used directly for biochemical assays. The widely used wheat germ cell-free protein expression method provides broad applications to investigate the function and structure of eukaryotic proteins. Such advantages, including high translation efficiency, flexibility, and possible automatization, are beneficial for achieving native-like chromatin substrates for in vitro studies.

Results

We describe a novel, single-step in vitro chromatin assembly method by using the wheat germ cell-free protein synthesis. We demonstrated that both Drosophila and human

SUBMITTER: Okimune KI 

PROVIDER: S-EPMC7708258 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature