Optogenetic intervention of seizures improves spatial memory in a mouse model of chronic temporal lobe epilepsy.

ABSTRACT:

Objective

To determine if closed-loop optogenetic seizure intervention, previously shown to reduce seizure duration in a well-established mouse model chronic temporal lobe epilepsy (TLE), also improves the associated comorbidity of impaired spatial memory.

Methods

Mice with chronic, spontaneous seizures in the unilateral intrahippocampal kainic acid model of TLE, expressing channelrhodopsin in parvalbumin-expressing interneurons, were implanted with optical fibers and electrodes, and tested for response to closed-loop light intervention of seizures. Animals that responded to closed-loop optogenetic curtailment of seizures were tested in the object location memory test and then given closed-loop optogenetic intervention on all detected seizures for 2 weeks. Following this, th

SUBMITTER: Kim HK 

PROVIDER: S-EPMC7708390 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature