SATB1 promotion of trophoblast stem cell renewal through regulation of threonine dehydrogenase.

ABSTRACT:

Background

Trophoblast stem (TS) cell renewal and differentiation are essential processes in placentation. Special AT-rich binding protein 1 (SATB1) is a key regulator of the TS cell stem state. In this study, we identified SATB1 downstream targets and investigated their actions.

Methods

RNA-sequencing analysis was performed in Rcho-1 TS cells expressing control or Satb1 short hairpin RNAs (shRNAs) to identify candidate SATB1 targets. Differentially regulated transcripts were validated by reverse transcription-quantitative polymerase chain reaction. The role of a target of SATB1, L-threonine 3-dehydrogenase (TDH), in the regulation of trophoblast cell development was investigated using a loss-of-function approach.

Results

Among the differentially regulated transcripts

SUBMITTER: Kubota K 

PROVIDER: S-EPMC7708522 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature