Bringing the Heavy Chain to Light: Creating a Symmetric, Bivalent IgG-Like Bispecific.

ABSTRACT: Bispecific molecules are biologically significant, yet their complex structures pose important manufacturing and pharmacokinetic challenges. Nevertheless, owing to similarities with monoclonal antibodies (mAbs), IgG-like bispecifics conceptually align well with conventional expression and manufacturing platforms and often exhibit potentially favorable drug metabolism and pharmacokinetic (DMPK) properties. However, IgG-like bispecifics do not possess target bivalency and current designs often require tedious engineering and purification to ensure appropriate chain pairing. Here, we present a near-native IgG antibody format, the 2xVH, which can create bivalency for each target or epitope and requires no engineering for cognate chain pairing. In this modality, two different variable heavy (VH

SUBMITTER: Ramasubramanian A 

PROVIDER: S-EPMC7709125 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature