Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation.

ABSTRACT:

Background

The ability to distinguish resident microglia from infiltrating myeloid cells by flow cytometry-based surface phenotyping is an important technique for examining age-related neuroinflammation. The most commonly used surface markers for the identification of microglia include CD45 (low-intermediate expression), CD11b, Tmem119, and P2RY12.

Methods

In this study, we examined changes in expression levels of these putative microglia markers in in vivo animal models of stroke, cerebral amyloid angiopathy (CAA), and aging as well as in an ex vivo LPS-induced inflammation model.

Results

We demonstrate that Tmem119 and P2RY12 expression is evident within both CD45^int and CD45^high myeloid populations in models of stroke, CAA, and aging. Interest

SUBMITTER: Honarpisheh P 

PROVIDER: S-EPMC7709276 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature