Detection of CRISPR-mediated genome modifications through altered methylation patterns of CpG islands.

ABSTRACT:

Background

The development and application of CRISPR technologies for the modification of the genome are rapidly expanding. Advances in the field describe new CRISPR components that are strategically engineered to improve the precision and reliability of CRISPR editing within the genome sequence. Genome modification using induced genome breaks that are targeted and mediated by CRISPR components leverage cellular mechanisms for repair like homology directed repair (HDR) to incorporate genomic edits with increased precision.

Results

In this report, we describe the gain of methylation at typically hypomethylated CpG island (CGI) locations affected by the CRISPR-mediated incorporation of donor DNA using HDR mechanisms. With characterization of CpG methylation patterns using whol

SUBMITTER: Farris MH 

PROVIDER: S-EPMC7709351 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature