Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of <i>Treponema pallidum</i> and <i>Haemophilus ducreyi</i> in Yaws-Like Lesions.

ABSTRACT: Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effective diagnosis is pivotal in the successful implementation of this control program. Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification technique offers a wider range of differentiation of pathogens including those isolated from chronic skin ulcers with similar characteristics such as Haemophilus ducreyi (H. ducreyi). We have developed a RPA assay for the simultaneous detection of Treponema pallidum (T. pallidum) and H. d

SUBMITTER: Frimpong M 

PROVIDER: S-EPMC7709673 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature